Macromolecular Crystallography

Overview

• Excellent brief online overview, by Phil Jeffrey at Princeton
• Protein Crystallography, by S.E.Sedelnikova
• Protein Crystallography Universe

Books

• Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology, by Bernard Rupp
• Introduction to Macromolecular Crystallography, by Alexander McPherson

Crystallization

• Crystallization tips:
  • Protein crystallization: from purified protein to diffraction-quality crystal. (Chayen and Sardakis, 2008, Nature Methods, 5, p147)
  • Hampton Research Tips and Crystal Growth 101
  • Emerald BioSystems Protein Crystallization Hits
  • Pictoral library of drops from Terese Bergfors at USF
  • Crystals Wiki

Crystal Looping & Freezing

• Pictures of cryo tools and loop/cap/vial identification
• Pcitures of implements for crystal storage
• Cryoprotection of delicate crystals, by Artem G. Evdokimov
• Looping supplies:
  • Hampton Research nylon loops
  • MiTeGen polymer MicroMounts

Data Collection, Processing, Solution, & Refinement

• Crystal diffraction screening tips from Structural Medicine at the U of Cambridge
• Part Four: Data Collection Strategy from the Structural Molecular Biology Laboratory at UCLA
• XRayView, from the Phillips lab at U of Wisc-Madison: excellent interactive graphic tutorial on X-ray diffraction concepts
• Links to data processing manuals at SSRL
• XDS by Wolfgang Kabsch from Max Planck Institute
• CCP4 for structure solution and refinement
• Phenix for automated structure determination and refinement
• SHELX for crystal structure determination
• SBGrid: crystallography software programs available at Caltech

SSRL beamline

• Macromolecular Crystallography at SSRL
• Operating Schedule
• Webice Login Page
• Data collection and processing
• SSRL FAQ
• Referencing SSRL

Forms

• Form for using Mosquito and Griffins to set up crystal trays
• Table for keeping track of Crystals
• Table for keeping track of Datasets